Composite

Part:BBa_K3799007

Designed by: Lakshmi Prakash   Group: iGEM21_IISER_Kolkata   (2021-10-01)


Unidirectional model of AIP-1 sensor of S aureus regulating GFP intensity using P2 promoter

This part consist of the AgrC-AgrA quorum-sensing signal transduction system of S.aureus regulated by pBad promoter from E. Coli(BBa_K206000).The activity of this two-component system in turn regulate P2 promoter which is studied using GFP expression(BBa_K391000)


The regulation of P2 promoter by the two-component system when both Pveg and P2 promoter are aligned in the same direction is studied using this system. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2953


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